Fig. 4: Anti-human PAR4 antibody responses following PAR4-mRNA-LNP immunization.
After immunizing BALB/c mice (n = 6) with PAR4-mRNA-LNP through intramuscular injection, a PAR4-expressing cell-based ELISA was used to measure the anti-PAR4 antibody responses with horseradish peroxidase (HRP)-conjugated anti-mouse IgG antibody for detection. A Following the 2nd boost, two mice exhibited anti-PAR4 antibody responses. B After the 3rd boost, five mice produced anti-PAR4 antibody responses. C Following the final boost, all mice exhibited anti-PAR4 antibody responses; specifically, mice 1, 2, 4, 5, and 6 displayed extremely high PAR4 antibody responses, even at a 30,000-fold dilution.
