Fig. 5: PAR4-expressing cell-based ELISA to evaluate anti-PAR4 antibody from hybridoma cells. | npj Vaccines

Fig. 5: PAR4-expressing cell-based ELISA to evaluate anti-PAR4 antibody from hybridoma cells.

From: To generate functional anti-protease-activated receptor-4 (PAR4), a G protein-coupled receptor, antibodies through PAR4-mRNA-LNP immunization

Fig. 5: PAR4-expressing cell-based ELISA to evaluate anti-PAR4 antibody from hybridoma cells.

Fifteen clones were screened using a PAR4-expressing and 293 T alone cell-based ELISA. A The anti-PAR4 antibodies were measured using HRP-conjugated anti-mouse IgG + IgM. B The HRP-conjugated anti-mouse IgG Fc fragment identified the isotypes of the anti-PAR4 antibodies in the supernatants from each hybridoma cell. Thirteen hybridoma clones produced high levels of specific anti-PAR4 IgG (10 clones) or IgM (3 clones). Anti-PAR4 Ab served as a positive control.

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