Fig. 3: Serological response to vaccination by ELISA.

a Schematic illustrating immunizations at weeks 0 and 5. Sera was taken from 1 week after last injection and challenge with live sporozoites was done at either 2 weeks or 5 weeks after last injection. Liver fluorescence was imaged at 42 h after challenge time. b Binding of sera to J2 peptide coated plates. Symbols represent area under the curve (AUC) values for individual mice (n = 6). When comparing serological responses between 24mers and 60mers displaying same peptides, significance testing was performed using a two-tailed Mann-Whitney U test; ns not significant; **p < 0.005. c Binding of sera to wild-type C-terminal domain captured on ELISA plates coated with either anti-alpha site antibody 236 or anti-beta site antibody 1512. Symbols represent AUC values for individual mice (n = 10); some groups have fewer than 10 data points due to insufficient sera. d Binding of sera to full-length N-terminal domain coated plates. Symbols represent AUC values for individual mice (n = 10). Groups were taken from different mouse trials.