Fig. 5: Structures of four representative Fabs in complex with NiV G^H.

A Cryo-EM structure of G^H bound simultaneously with Fabs of S1E2, S2B10 and LN3D3 is shown as a cartoon representation. B Crystal structure of LN1F9 Fab complexed with G^H is shown as cartoon. The heavy and light chain variable domains of S1E2, S2B10, LN3D3 and LN1F9 are colored blue and skyblue, olive and wheat, red and salmon, green and palegreen, respectively. G^H is colored gray. C Surface representation of G^H with contact surfaces colored as in (A, B). D–G Delineation of the epitope contacts on the G^H for S1E2 (D), S2B10 (E), LN1F9 (F), and LN3D3 (G). Side chains are shown as sticks and labeled if they form hydrogen bonds, with nitrogen and oxygen atoms of the side chains colored magenta and black, respectively. G^H residues contacted by both chains are colored cyan. H The NiV_M G^H amino acid sequence with structurally defined epitopes highlighted as in (A–G).