Fig. 7: CD4+ T follicular helper cell (Tfh) responses in PBMCs and cytokine responses. | npj Vaccines

Fig. 7: CD4+ T follicular helper cell (Tfh) responses in PBMCs and cytokine responses.

From: ALFQ adjuvanted HIV-1 envelope protein vaccination elicits durable functional antibody and cellular responses in nonhuman primates

Fig. 7

The frequencies of A Env-specific Tfh cells (CXCR5+ PD-1hi CD4+ T cells), B Env-specific IL-21-secreting Tfh, and C percentage of CCR5+ CD4+ T cells at week 26 were determined by flow cytometry and the data are presented as mean ± SEM. Each individual NHP is represented as a symbol (N = 7/group). A representative flow plot is shown on the right. D Cytokines in culture supernatants of PBMCs at weeks 14 and 26 were analyzed by MSD. The data from each group is shown as Box and Whisker plots. E, F Heat map of cytokines and chemokines in the sera of individual animals 2 days post-first and second (weeks 12 and 24) protein vaccinations. Statistical significance between the groups was determined using a non-parametric Mann–Whitney two-tailed test. *p < 0.02. The flow panel and the gating strategy are provided in Fig. S5C and Table S9, respectively.

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