Fig. 2: In vivo identification of immunodominant T cell epitopes in ZIKV-infected mice. | npj Vaccines

Fig. 2: In vivo identification of immunodominant T cell epitopes in ZIKV-infected mice.

From: Multi-antigen DNA vaccine targeting non-structural proteins confers robust T Cell-mediated protection against Zika virus

Fig. 2: In vivo identification of immunodominant T cell epitopes in ZIKV-infected mice.

A Schematic of epitope mapping experiment. Female BALB/c mice (n = 5/group) were infected i.v. with 200PFU of ZIKVPRVABC59. On day 35 post-infection, mice were injected i.v. autologous splenocytes labeled with unique combinations of cell-tracking dyes (CTV, CFSE, CPD) and pulsed with 246 overlapping peptides spanning NS1, NS3, NS4, and Env regions previously identified as immunodominant pools. B CD8⁺ T cell cytotoxic responses were quantified using FTA. Mean ± SEM percentage specific killing of individual peptide-pulsed targets is shown, compared to uninfected controls. C CD4⁺ T cell responses were measured by CD69 upregulation on B220⁺ peptide-pulsed targets. Data are presented as GMFI ± SEM for each peptide cluster. Statistical comparisons were performed using two-way ANOVA with Tukey’s multiple comparisons test. **P < 0.01; ***P < 0.001.

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