Fig. 3: Functional avidity of ZIKV-specific CD8⁺ and CD4⁺ T cell responses. | npj Vaccines

Fig. 3: Functional avidity of ZIKV-specific CD8⁺ and CD4⁺ T cell responses.

From: Multi-antigen DNA vaccine targeting non-structural proteins confers robust T Cell-mediated protection against Zika virus

Fig. 3: Functional avidity of ZIKV-specific CD8⁺ and CD4⁺ T cell responses.

A Experimental timeline for peptide avidity analysis. Female BALB/c mice (n = 5/group) were infected with 200PFU of ZIKVPRVABC59 or left uninfected (orange). On days 7 (purple) or 35-days (red) post-infection, mice were injected intravenously with naive splenocyte targets pulsed with serial dilutions (10–0.01 μg/mL) of immunodominant peptides identified in Fig. 2. B CD8⁺ T cell cytotoxicity was quantified using the FTA assay. Data show mean ± SEM percentage specific killing at each peptide concentration for each target cluster (C) CD4⁺ T cell activity was assessed by CD69 expression on B220⁺ target cells pulsed with NS1202-216 and NS1205-219 peptides. Data represent GMFI ± SEM in ZIKV-infected and uninfected mice. Statistical comparisons using two-way ANOVA with Dunnett’s post-hoc (vs. uninfected controls). * P < 0.05, **P < 0.01, ***P < 0.001.

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