Fig. 5: aroA contributes to S. agalactiae endothelial cell damage, blood-brain barrier disruption and induction of neuroinflammation. | npj Vaccines

Fig. 5: aroA contributes to S. agalactiae endothelial cell damage, blood-brain barrier disruption and induction of neuroinflammation.

From: Evaluation of safety and efficacy of an auxotrophic aroA mutant live attenuated vaccine against piscine Streptococcus agalactiae infection

Fig. 5: aroA contributes to S. agalactiae endothelial cell damage, blood-brain barrier disruption and induction of neuroinflammation.

A Adhesion ability of the indicated strains to bEnd.3 cells. At 2 h postinfection, cells were washed and lysed for measurement of the number of CFU. B Bacterial cytotoxicity assessed by LDH release from RAW 264.7 macrophages after 4 h infection. C Evans Blue (EB) dye accumulation at 16 and 72 hpi to evaluate the permeability of the BBB (n = 3). Cerebral transcription levels of cytokines IL-1β (D), IL-6 (E), and TNF-α (F) in BALB/c mice infected with the indicated S. agalactiae strains (n = 5). Error bars represent SD. *P < 0.05; **P < 0.01; ***P < 0.001.

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