Fig. 4: CD8 + T cell responses to RV vaccination in infants compared to CMV. | npj Vaccines

Fig. 4: CD8 + T cell responses to RV vaccination in infants compared to CMV.

From: Peripheral rotavirus-specific T-cell responses following monovalent oral rotavirus vaccine in infants

Fig. 4: CD8 + T cell responses to RV vaccination in infants compared to CMV.The alternative text for this image may have been generated using AI.

A Representative flow cytometry plots showing the gating strategy for determining AIM + CD8 + T cell response using Boolean OR gating. B Paired analysis of pre- vs post-vaccine magnitudes of antigen-specific CD8 + T cells identified by Boolean gating. Data are shown with median, quartiles and minimum/maximum values. Magnitudes represent the proportion of RV- (n = 26) or CMV-specific (n = 14) CD8 + T cells - the DMSO vehicle control expressed as a % of total CD8 + T cells (C) Matched pairwise comparisons of pre- vs post-vaccine RV-specific CD8 + T cells in IgA responders (n = 11) and non-responders (n = 15). Median FC values are displayed. D Representative flow cytometry plots showing the gating strategy for the identification of functional responses in RV- and CMV-specific CD8 + T cells. E Unpaired analysis of functional responses of PHA (black), RV (blue) and CMV (red) stimulated CD8 + T cells in T cell responders (n = 11). Data are shown with the mean and standard error of the mean. F, G Unpaired analysis of CD25+ IFNy+ and CD25+ Granzyme B+ response magnitudes in RV- (n = 12) and CMV-specific (n = 14) CD8 + T cells in T cell responders expressed as a % of total CD8 + T cells. Data are shown with the mean and standard error of the mean. Matched pairwise comparisons in B and C were made by Wilcoxon matched-pairs signed rank tests. Comparisons between IgA responders and non-responders at pre- and post-vaccine and comparison between RV and CMV phenotypes were made by Mann-Whitney. Comparisons across multiple unmatched groups were made by Kruskal-Wallis tests corrected for multiple comparisons by Dunns post hoc test. Data points represent individual infants. Horizontal lines represent the limit of sensitivity (LOS) for each assay as determined by the median + 2 x standard deviation of a synthetic non-specific myelin oligodendrocyte glycoprotein (MOG) peptide response (B, C = 0.029, E IFN + = 0.012, TNFa + = 0.0031, IFN + /TNFa + = 0.0018, F = 0.012, G = 0.069). PHA phytohemagglutinin-L, RV rotavirus, CMV cytomegalovirus, FC fold change, DMSO dimethyl sulfoxide, R RV vaccine serologic responder, NR RV vaccine serologic non-responder.

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