Fig. 2: Structural design and modeling of the MEU–Flagellin fusion protein.

A Schematic representation of the MEU–Flagellin construct comprising sixteen predicted B- and T-cell epitopes derived from six bacterial proteins. The N- and C-terminal domains of Salmonella flagellin were fused to the respective termini of the MEU sequence. B Predicted secondary structure showing predominantly α-helical flagellin N- and C-terminal domains and a structurally diverse MEU region composed of loops, short helices, and β-strands. C Three-dimensional structural model illustrating elongated α-helical flagellin arms transitioning into a compact, folded MEU domain.