Fig. 3: A-910823 directly induced IL-1α and IL-1β. | npj Vaccines

Fig. 3: A-910823 directly induced IL-1α and IL-1β.

From: IL-1 delineates squalene-based adjuvant efficacy and reactogenicity in a cell-type-specific manner

Fig. 3: A-910823 directly induced IL-1α and IL-1β.The alternative text for this image may have been generated using AI.

A Chemical structure of α-tocopherol covalently bound with BODIPY. B Fluorescence intensity of A-910823 and A-910823 containing fluorescently labeled α-tocopherol (A-910823-BODIPY) confirmed by fluorescence spectrum. C57BL/6 J mice were injected IM with Ag mixed with A-910823 or A-910823-BODIPY (n = 3/group). C Representative BODIPY luminance of eosinophils, neutrophils, monocytes, and (DCs) 6 h post-administration. Mean fluorescence intensity (MFI) of D IL-1α and E IL-1β in BODIPY-positive and -negative cells, respectively. Bars represent means; error bars indicate standard error of the mean. Statistical significance determined using two-way ANOVA. ns, P ≥ 0.05, *P < 0.05, ***P < 0.001, ****P < 0.0001.

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