Table 2 Data summary and performance of the AT method compared with the reference test.

From: Novel methods for global water safety monitoring: comparative analysis of low-cost, field-ready E. coli assays

E. coli count (cfu 100 ml−1) risk category from reference methoda

AT, standard temperature incubation

n = 945

AT, ambient temperature incubation

n = 945

100 ml

10 ml

100 ml

10 ml

 

+

+

+

+

<1 (n = 334)

14

320

1

333

11

323

0

334

1–10 (n = 123)

117

6

0

123

118

5

0

123

11–100 (n = 245)

235

10

46

199

230

15

43

202

101+ (n = 243)

241

2

205

38

243

0

209

34

 

Test accuracy statistics

Sensitivity: presence of E. coli (95% CI)a,b

97.1% (95.4–98.2%)

51.4% (46.9–56.0%)

96.7% (95.0–98.0%)

51.6% (47.1–56.2%)

Specificity: presence of E. coli (95% CI)a,b

95.8% (93.1–97.7%)

99.8% (98.8–100%)

96.7% (94.2–98.3%)

100% (99.2–100%)

Positive predictive value (PPV): presence of E. coli (95% CI)a,b

97.7% (96.2–98.6%)

99.6% (97.3–99.9%)

98.2% (96.8–99.0%)

100%

Percentage of total samples underestimating risk category a,b

1.9%

25%

2.1%

25%

Percentage of total samples overestimating risk category a,b

1.5%

0.11%

1.2%

0%

  1. aUsing the following thresholds: <1 cfu 100 ml−1 for 100 ml test and 11 cfu 100 ml−1 for 10 ml test.
  2. bConfidence intervals for sensitivity and specificity are “exact” Clopper–Pearson confidence intervals30; for PPV, these are standard logit confidence intervals31.