Fig. 6: The response surface plots showing the effects of variables on extraction of MG.

a The interaction of ultrasonication time and pH of solution, b the interaction of ultrasonication time and extraction solvent volume, c the interaction of ionic strength and ultrasonication time (experimental conditions: ionic strength: 0.0–10.0 w/v%, extraction solvent: chloroform (50–250 µL), sonication time: 1–5 min, dispersive solvent: ethanol (200–1000 µL), pH: 3–7, water sample volume: 10 mL, centrifuge rate: 3500 rpm, spiked concentration: 500 ng mL−1).