Fig. 3: Accessory genome clustering and virulence and antibiotic resistance gene screening of wastewater and clinical ExPEC strains.

Binary accessory gene presence–absence clustering tree A of all original presumptive wastewater ExPEC strains (n = 86) and including those closely matching clinical ExPEC strains (n = 37 of the 86), as well as clinical ExPEC strains, naturalized wastewater E. coli strains, and laboratory reference strains. Included in this analysis are the number of antibiotic resistance genes (ARG) and virulence genes (VG) present in each isolate (B). The major sequence types represented are indicated in highlighted boxes in the clustering tree. Any original presumptive wastewater ExPEC strains that did not exhibit high genetic similarity with a clinical ExPEC strain at the whole and core genome levels are indicated in the clustering tree with an asterisk (*).