Fig. 1: SHERLOCK detection of SARS-CoV-2 RNA. | Nature Biomedical Engineering

Fig. 1: SHERLOCK detection of SARS-CoV-2 RNA.

From: Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA

Fig. 1

A SARS-CoV-2 RNA region of interest is isothermally amplified to DNA by RT–RPA, then converted to RNA by T7 transcription. Cognate binding of Cas13a–crRNA complex to amplified RNA targets triggers collateral activity of Cas13a, which cleaves RNA reporters. Cleaved RNA reporters can be captured on a colorimetric lateral-flow strip (biotin–fluorescein RNA reporter, top path) or visualized by fluorescence signal (molecular beacon fluorescent reporter, bottom path). Fl, fluorescein.

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