Fig. 3: Specificity of SHERLOCK SARS-CoV-2 S gene detection.
From: Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA
a, Alignment of the S gene region of SARS-CoV-2 with other common human coronaviruses, including hCoV-229E, hCoV-NL63, hCoV-HKU1 and hCoV-OC43. Nucleotides of RPA-primer- and crRNA-binding regions of the SARS-CoV-2 S gene (highlighted in pink) that are common between SARS-CoV-2 and the other viruses are highlighted in grey. b, Kinetics of fluorescence signal generation of the CRISPR–Cas13a reaction in RNA extracts from clinical samples tested positive with different coronaviruses, using SHERLOCK SARS-CoV-2 S gene detection. c, Fluorescence signals generated after 60 min of CRISPR–Cas13a reaction for each virus. Negative control is RNA extracted from a SARS-CoV-2-negative (by RT–qPCR) clinical sample.
