Fig. 3: Multiplexed antibody assay against various antigens of novel and previous coronaviruses. | Nature Biomedical Engineering

Fig. 3: Multiplexed antibody assay against various antigens of novel and previous coronaviruses.

From: Quantification of antibody avidities and accurate detection of SARS-CoV-2 antibodies in serum and saliva on plasmonic substrates

Fig. 3: Multiplexed antibody assay against various antigens of novel and previous coronaviruses.

a, Correlation plot of normalized anti-S1 IgG level (y axis) and normalized anti-RBD IgG level (x axis) measured in PCR-confirmed COVID-19 sera (n = 136). The dashed line is drawn to have a slope of 1. The upper left inset (outlined in green) shows a scanned image of the IgG-only channel in a patient serum labelled as PAMF-065, which displays high signal on the S1 antigen but not on the RBD antigen. The lower right inset (outlined in orange) shows a scanned image of IgG levels of a sample labelled as PAMF-011, displaying approximately equal IgG signals against S1 and RBD. b, Correlation plot of normalized anti-S1 IgM level (y axis) and normalized anti-RBD IgM level (x axis) measured in COVID-19 PCR-positive sera (n = 136). The dashed line is drawn to have a slope of 1. c, MFI of IgG detected in COVID-19 PCR-positive sera (n = 62) against S1 of SARS-CoV-2, SARS-CoV-1 and four common human coronaviruses (HCoV) 229E, HKU1, NL63 and OC43, and against RBD of SARS-CoV-2 and SARS-CoV-1 only. d, The same as c, but for pre-pandemic sera (n = 40). The raw data corresponding to a and b are provided in Supplementary Table 7. Data in c and d are presented as box plots with the minima, maxima, median line, interquartile range, data points and outliers shown.

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