Extended Data Fig. 3: The surface proteome is unaltered by the dual functionalization of engineered EVs. | Nature Biomedical Engineering

Extended Data Fig. 3: The surface proteome is unaltered by the dual functionalization of engineered EVs.

From: Amelioration of systemic inflammation via the display of two different decoy protein receptors on extracellular vesicles

Extended Data Fig. 3: The surface proteome is unaltered by the dual functionalization of engineered EVs.

A) Imaging flow cytometry analysis (IFCM) with dot plots and example event images in the double positive (DP) gate of MSC TNFR1∆∆-FDN-NST and MSC double decoy EVs stained with mIL6ST APC conjugated and hTNFR1 PE conjugated antibody. PBS + antibodies were used for background adjustment and for determining the gating strategy. B) Multiplex EV surface characterization of PAN (CD63, CD81, and CD9) positive, hTNFR1 positive and mIL6ST positive population in MSC double decoy EVs and MSC Ctrl EVs. Data represented as background corrected median APC fluorescence intensity determined by flow cytometry of EVs bound different capture beads and upon using APC labelled detection antibody. Central Value: Mean, Error bars: S.D, (Technical replicate, n = 5000-15000).

Back to article page