Extended Data Fig. 3: Autofluorescence in diabetic human kidney tissue imaged with MediSCAPE.

Autofluorescence captured by MediSCAPE reveals features common to and beyond those seen on routine histology. a, PAS histology image of kidney cortex tissue from an older, diabetic patient with features of mild diabetic nephropathy. Scale bar, 200 µm. b, a MediSCAPE xy slice from a stage-scanned volume of the same piece of tissue (while fresh) showing autofluorescence excited at 488 nm. Yellow arrows point to the kidney capsule, white arrows indicate urinary casts, seen in both SCAPE and PAS images. Dotted line squares indicate regions of interest shown in more detail in c, highlighting features more evident by SCAPE than PAS histology. Scale bar, 200 µm. c(i), A focal subcapsular collection of tubules with autofluorescent cytoplasmic granules (teal arrow). Urinary cast material (white arrows) is also evident in the xy plane, and further evidenced by characteristically strong autofluorescence in the yz plane. c(ii). Tubules with accentuated peritubular autofluorescence (red arrow). c(iii) Glomerulus with focal autofluorescent granules. Scale bars, 50 µm.