Extended Data Fig. 2: Label-free imaging of fresh mouse tissue with miniaturized MediSCAPE.

xy (top) and yz (bottom) cross-sections in various fresh mouse tissue acquired with the miniaturized MediSCAPE system with 488 nm excitation and dual colour emission channels labelled as shown. Cross- sections show a, tubules in the kidney cortex, b, capsule and underlying cords of hepatocytes in the liver, c, cardiac muscle in the heart and d, crypts of Lieberkühn in the colon mucosa. White dotted lines in the xy planes indicate the location of corresponding yz planes shown. e. 3D rendering (Imaris) of a stitched field of view created from 16 seconds of continuous volume acquisition at 11.2 VPS while roving across the colon mucosa. Insets to the right show single planes from the ROI indicated (yellow dotted box). Yellow dotted lines in the xy cross-section show the locations of corresponding depth cross- sections. Only emission at ~525 nm is shown here, in grayscale. All scale bars, 100 µm.