Fig. 5: HCMV detection by suboptimal PAM-mediated one-pot reaction.
a–d, The sensitivity of suboptimal PAM-mediated one-pot reaction (b,d) and qPCR assay (a,c) targeting the UL55 gene of HCMV was compared. The PUC57-UL55 plasmid was used as substrate in a and b. The presence of HCMV virus was determined in c and d. The reaction volume of qPCR in a and c is 20 μl and the reaction volume of one-pot reactions in b and d is 30 μl. The numbers of copies inputted in the two reactions were the same. e, Schematic of detection under portable UV light and using a lateral flow strip. The lysed viral samples were used as an input to sPAMC, which was visualized by UV light or lateral flow strip. f, The direct fluorescence stimulated by UV light was visualized to detect HCMV virus. The reaction was examined under UV light at 8, 10, 15 and 20 min after incubation at 37 °C. g, At 20 min after incubation, a lateral flow strip was dipped into the reaction tube for 5 min to visualize the control and test bands. Red arrows indicate the flow direction of samples in strips.
