Extended Data Fig. 1: Suboptimal and canonical PAMs-mediated one-pot reactions. | Nature Biomedical Engineering

Extended Data Fig. 1: Suboptimal and canonical PAMs-mediated one-pot reactions.

From: Fast and sensitive detection of SARS-CoV-2 RNA using suboptimal protospacer adjacent motifs for Cas12a

Extended Data Fig. 1: Suboptimal and canonical PAMs-mediated one-pot reactions.

a,b, One-pot reactions used spacers with suboptimal or canonical PAMs in Orf1ab (a) and E (b) genes of SARS-CoV-2. The crRNAs 1–3 targeting Orf1ab gene and crRNAs 2–7 targeting E gene used canonical PAMs whereas crRNAs 4-5 targeting Orf1ab gene and crRNA 1 targeting E gene used suboptimal PAMs. c, The PAMs and spacers used in the one-pot reaction.

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