Extended Data Fig. 2: Determination of L. lactis load in the mouse intestine.
From: An engineered live biotherapeutic for the prevention of antibiotic-induced dysbiosis
a. Treatment regimen. Mice were orally gavaged with two doses of 1010 CFU of L. lactis. The first dose occurred 2 hours prior to the ampicillin injection and the second simultaneous with the 200 mg/kg ampicillin injection. Intestinal load was evaluated each day for L. lactis spTEM1 and the empty-vector control strain L. lactis EV. b. Enumeration of viable L. lactis cells in feces in each day of treatment demonstrates no difference in the fecal loads of L. lactis spTEM1 (n = 8) and L. lactis EV (n = 8). Box plots display minimum, 25th percentile, median, 75th percentile, and maximum of biological replicate measurements. Unpaired two-sided Wilcoxon test: Day 1, p = 0.7944; Day 2, p = 0.0511; Day 3, p = 0.4093.
