Extended Data Fig. 1: Kinesin-2 is dispensable for hPSC morphology and epiblast spheroid formation. | Nature Biomedical Engineering

Extended Data Fig. 1: Kinesin-2 is dispensable for hPSC morphology and epiblast spheroid formation.

From: Modelling ciliopathy phenotypes in human tissues derived from pluripotent stem cells with genetically ablated cilia

Extended Data Fig. 1

a, Confocal immunofluorescence images of acetylated a-tubulin (AcTub) and DNA in representative fields of undifferentiated control and KIF3A-/- hPSCs. Orthogonal (top) and volume (bottom) views are shown. b, Phase contrast images of representative control and KIF3A-/- epiblast spheroids. c, Quantification (mean ± s.e.m.) of lumen area as percentage of the spheroid area. d-e, Confocal sections showing immunofluorescence for pluripotency, polarity, and ciliary markers in spheroids. A midbody is seen in a KIF3A-/- spheroid (arrow), but not cilia. Scale bars, 25 µm.

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