Extended Data Fig. 3: Characterization of tissue specific maturation.
From: A multi-organ chip with matured tissue niches linked by vascular flow
a-c, Electromechanically matured cardiac tissues show aligned actinin alpha expression (green, scale bar, 10 µm) (a), functional improvements in maximum capture rate and excitation threshold (b) (n = 8-9 biological replicates), and increased force responses when exposed to increasing calcium concentrations (c) (n = 9 biological replicates). d-e, Liver tissues are matured via co-culture of cells in 3D aggregated as detailed by immunohistochemical staining (d) and increased albumin secretion (e) (n = 6 biological replicates). f, Immunohistochemical staining of engineered bone slices stain positive for osteocalcin, TRAP activity, and bone sialoprotein. g, Micro-computed tomography imaging of bone scaffolds over time details bone remodeling during the osteoblastic and osteolytic phases of induced bone maturation (n = 13 biological replicates). h, Immunohistochemical staining of engineered skin slices detail the formation of the epidermis and dermis over 4 weeks of maturation. i, TEER values detail the barrier function of the engineered skin, with reported values detailed by the red shaded region23 (n = 15 biological replicates). Data is shown as mean ± SD and statistics determined by unpaired t-test (b, c, e), or one-way ANOVA (g, i).
