Extended Data Fig. 4: M-L6-IL12 and unmodified IL-12 induce similar expression of proinflammatory markers and cell infiltration in B16F10 melanoma. | Nature Biomedical Engineering

Extended Data Fig. 4: M-L6-IL12 and unmodified IL-12 induce similar expression of proinflammatory markers and cell infiltration in B16F10 melanoma.

From: Masking the immunotoxicity of interleukin-12 by fusing it with a domain of its receptor via a tumour-protease-cleavable linker

Extended Data Fig. 4: M-L6-IL12 and unmodified IL-12 induce similar expression of proinflammatory markers and cell infiltration in B16F10 melanoma.The alternative text for this image may have been generated using AI.

Mice were treated as described in Fig. 3. Intratumoral levels of CXCL-1 (a), CCL-11 (b), CCL-17 (c), IL-10 (d), IL-12 (e), IL-6 (f) were quantified using a LEGENDPlex assay and normalized by total tumor protein content. Frequency of CD3+CD4+Foxp3− T cells (g), CD11c+MHCII+ dendritic cells (h), and CD11b+F4/80+ macrophages (i) as percentage of live cells are shown. PBS, n = 8; IL-12, n = 8; M-L6-IL12, n = 7. Data are mean ± s.e.m. Statistical analyses were performed using ordinary one-way ANOVA with Tukey’s multiple comparison test. Experiment was performed twice with similar results.

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