Extended Data Fig. 5: Masked IL-12 minimizes systemic inflammatory response in healthy mice. | Nature Biomedical Engineering

Extended Data Fig. 5: Masked IL-12 minimizes systemic inflammatory response in healthy mice.

From: Masking the immunotoxicity of interleukin-12 by fusing it with a domain of its receptor via a tumour-protease-cleavable linker

Extended Data Fig. 5

Mice were treated and analyzed as described in Fig. 4b-h. Plasma levels of IL-12 (a), IL-10 (b), IL-1a (c), IL-1b (d), and IFNb (e) were quantified using a LEGENDPlex assay. Serum levels of albumin (f), blood urea nitrogen (g), and total protein (h) were quantified using a blood chemistry analyzer. PBS, n = 5; IL-12, n = 7; M-L6-IL12, n = 6. Data are mean ± s.e.m. Statistical analyses were performed using ordinary one-way ANOVA with Tukey’s multiple comparison test. Experiments were performed twice with similar results. L.O.D = limit of detection.

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