Extended Data Fig. 7: DP TRAC-1XX-iT cell mature to CD8αβ SP iT cells on 3T3-CD19-41BBL. | Nature Biomedical Engineering

Extended Data Fig. 7: DP TRAC-1XX-iT cell mature to CD8αβ SP iT cells on 3T3-CD19-41BBL.

From: Generation of T-cell-receptor-negative CD8αβ-positive CAR T cells from T-cell-derived induced pluripotent stem cells

Extended Data Fig. 7

a, c, Flow cytometric analysis of D42 cells matured on 3T3-CD19 (a) or 3T3-CD19-41BBL (c). Gated on live CD45+CD7+ cells. b, Flow cytometric analysis of D35 and D42 phenotypes of stimulated DP TRAC-1XX-iT cells. D35 TRAC-1XX-iT cells were sorted for a CD4+CD8αβ+ DP phenotype, stimulated on 3T3-CD19-41BBL and expanded for seven days. Gated on live CD45+CD7+ cells. d, Fold Expansion and T cell phenotype marker expression of TRAC-1XX-iT cells matured on 3T3-CD19-41BBL (3T3) or recombinant CD19-Fc. e, 4 h cytotoxicity assay of 3T3-CD19-41BBL stimulated TRAC-1XX-iT cells in response to NALM6-CD19+ (red) an NALM6-CD19–/– (blue) as target cells (n = 3 technical replicates, data are means ± s.d.) f, CD19 expression on primary CLL cells. Filled grey histogram are unstained CLL cells, open red histogram are stained CLL cells.

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