Fig. 2: Expression of human IgG1 protein in transgenic minipigs.
From: A humanized minipig model for the toxicological testing of therapeutic recombinant antibodies
a, Western blot showing expression of IgG heavy γ1 (IgH-γ1) and κ light (IgL-κ) proteins in serum isolated from the transgenic founder (TG1–-TG3) IgG minipigs and F1 animals from line TG2 and TG3. WT Göttingen minipigs were negative for the presence of both proteins. Human IgG protein and PBS were used as positive and negative control, respectively. Unprocessed images of all western blots are shown in Supplementary Data 1. b, The presence of human IgG1 protein was further confirmed by ELISA analysis in serum isolated from F1 minipigs representing all 3 lines (TG line 1, n = 5; TG line 2, n = 4; TG line 3, n = 3). No human IgG1 was detected in WT animals (n = 4). Horizontal bar represents median. Each data point represents a biological replicate. c, Human IgG heavy and κ light chain genes undergo functional gene rearrangements. A selection of rearrangements of all four VH and the two Vκ transgenes is depicted. Human VH- and VΚ-gene rearrangements show N nucleotide additions. Human IgG4 sequence of isotype switch variants is shown in brown. The J elements in the rearranged V genes are given in parenthesis, identified D gene elements are underlined and indicated at right.
