Fig. 2: Cis- versus trans-UCI conjugation. | Nature Biomedical Engineering

Fig. 2: Cis- versus trans-UCI conjugation.

From: Unbiased discovery of autoantibodies associated with severe COVID-19 via genome-scale self-assembled DNA-barcoded protein libraries

Fig. 2

a, IVT-RNA encoding TRIM21 or GAPDH with their distinct UCI barcodes was translated before or after mixing at a 1:1 ratio. qPCR analysis of the IPs using UCI-specific primers, reported as fold change versus IP with healthy control (HC) plasma, which is set to 1, when the IVT-RNA was mixed post-translation. Sjögren’s syndrome, SS. b, IVT-RNA encoding TRIM21 (black UCI) and GAPDH (grey UCI) were mixed 1:1 into a background of 100-fold excess GAPDH (white UCI) and then translated as a mock library. Sequencing analysis of the IPs, reported as fold change versus the HC IP of the 100× GAPDH. c, hORFeome MIPSA library containing spiked-in TRIM21, immunoprecipitated with SS plasma and compared with average of eight mock IPs (no plasma input). The TRIM21 UCI is shown in red. d, Relative fold difference of TRIM21 UCI in SS versus HC IPs, determined by sequencing.

Back to article page