Extended Data Fig. 7: Flow cytometry analysis of tumor-infiltrating lymphocytes (TILs). | Nature Biomedical Engineering

Extended Data Fig. 7: Flow cytometry analysis of tumor-infiltrating lymphocytes (TILs).

From: Variants of the adeno-associated virus serotype 9 with enhanced penetration of the blood–brain barrier in rodents and primates

Extended Data Fig. 7

a, Gating strategies for all panels. Lymphocyte populations were selected based on SSC-H and FSC-H parameters, followed by FSC-H and FSC-A gating to eliminate doublets. CD45 marks for all immune cells. b, Representative flow cytometry plots and quantification of T cells (CD3+/CD45+) and their subtypes (CD4+/CD3+ T cells, CD8+/CD3+ T cells, IFNg+/CD3+ T cells, Granzyme B+/CD8+ T cells, CD25+/Foxp3 + /CD4+ Tregs). n = 3-11 mice per group, mean ± s.e.m., one-way ANOVA with Tukey’s post-test.

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