Extended Data Fig. 1: Initial round of in vivo selection revealing potential candidates for further optimization. | Nature Biomedical Engineering

Extended Data Fig. 1: Initial round of in vivo selection revealing potential candidates for further optimization.

From: Variants of the adeno-associated virus serotype 9 with enhanced penetration of the blood–brain barrier in rodents and primates

Extended Data Fig. 1

a, Fluorescence images of sagittal brain sections in mice 21 days after intravenous injection of 1 × 1010 vg of single-stranded AAV-EF1α-H2B-RFP. White dots indicate RFP-positive cells. Scale bars: 1000 μm for whole brain section and 30 μm for insets. b, Fluorescence images of sagittal brain sections in mice 21 days after intravenous injection of 1 × 1011 vg of selected AAVs-EF1α-H2B-RFP. Scale bars: 1,000 μm for whole brain section and 300 μm for insets. c, Percentage of transduced brain cells and relative fold change of AAV transduction as quantified by counting transduced RFP-positive cells using ImageJ. n = 3 mice per group, mean ± s.e.m., one-way ANOVA with Tukey’s post-test.

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