Extended Data Fig. 5: Analysis of BS3-stabilized 2:1 complex of MetECD and Fc(aMD4)B3. | Nature Biomedical Engineering

Extended Data Fig. 5: Analysis of BS3-stabilized 2:1 complex of MetECD and Fc(aMD4)B3.

From: Designing receptor agonists with enhanced pharmacokinetics by grafting macrocyclic peptides into fragment crystallizable regions

Extended Data Fig. 5: Analysis of BS3-stabilized 2:1 complex of MetECD and Fc(aMD4)B3.The alternative text for this image may have been generated using AI.

a, Molecular weight analyzed by SDS-PAGE indicated 2:1 and 1:1 complex of MetECD and Fc(aMD4)B3. SDS-PAGE analysis was performed using 7.5% gels (blue, from Fig. 3b) and 3-10% gels (orange, from b). b, SDS-PAGE analysis of Met dimer formation by Fc(aMD4) in vitro. The complexes between MetECD and Fc(aMD4) or control Fc were crosslinked by BS3 and analyzed by SDS-PAGE (3-10% gel) under non-reducing conditions and silver stained. c, MetECD and Fc(aMD4)B3 were crosslinked by BS3 and subjected to size exclusion chromatography (SEC). The results of non-reducing SDS-PAGE of the SEC fractions with Coomassie brilliant blue staining are shown. The fraction indicated in red was subjected to HS-AFM as shown in Fig. 3e, f.

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