Extended Data Fig. 8: The procedure of stereotactic biopsy in human glioma and the downstream analysis.
a: The placement of stereotactic frame from the frontal and lateral view. b: The illustration of trajectory for the biopsy entry point and the target on the MRI. c: The stereotactic biopsy platform. d, e: The view of aspiration side window cutting needle (d) and the acquired sample (e). f-i: examples of the downstream analysis for H&E, here, n = 35. Scale bar 50 µm. (f), IEM, here, n = 5. Scale bar 0.1 µm. (g), IHC, here, for AQP4, n = 45, for ZEB1, n = 10. Scale bar 50 µm. (h), and FACS (i) two biopsy points were obtained from one glioma patient, one sample for C6 TMZ 7 day. Scale bar 100 µm.
