Extended Data Fig. 2: CLIP is robust to protocol perturbation in primary T cells. | Nature Biomedical Engineering

Extended Data Fig. 2: CLIP is robust to protocol perturbation in primary T cells.

From: Stable expression of large transgenes via the knock-in of an integrase-deficient lentivirus

Extended Data Fig. 2

a, CLIP pseudovirus transduced into primary T cells 48 hours or 24 hours before electroporation of RNP showed minimal effect on transduction efficiency. b, 2.5×105, 5×105, or 1×106 cells were aliquoted into a cuvette for electroporation of Cas9-RNP, which had minimal effect on the knock-in efficiency. Data from one donor with technical duplicate.

Source data

Back to article page