Extended Data Fig. 6: Gene-delivery efficiency comparison of MC-SB/SB100X mRNA with the MAJESTIC system.
a, Flow-cytometry histogram overlays and bar plots of cell viability post-electroporation as measured with 7-AAD staining. b, Flow-cytometry data of CD22.CAR T cells from human primary CD3 T cells produced by plasmid transposon plasmid, transposon MC, transposon MC with mRNA-transposase, and MAJESTIC. c, Quantification of (b). d, Yield calculations (yield = CAR% * total viable cell count). All conditions started with an equal amount of primary T cells per replicate. Three CAR% replicates were averaged and then multiplied by the average of 2 cell count replicates. Left panel, total T cell count; Right panel, total CAR+ T cell count. e, Quantification of flow-cytometry data of CD22.CAR T cells from four human PBMCs (same data as Fig. 4e–f, plotted in dot-whisker plots).
