Fig. 4: Prediction of myopia onset using TRIPS-OCT in guinea pig model.

a,b, En face scleral birefringence images measured at the age of 2 weeks from the entire cohort of guinea pigs. Images are grouped by myopia outcome at the age of 4 weeks, defined as SE < 0D. Group 1 (a), myopic eyes. Group 2 (b), emmetropic and hyperopic eyes. Scale bar, 1 mm. c, PSB values measured from the images in the two groups of guinea pig eyes. Dots represent n = 42 eyes from 21 guinea pigs, central line indicates median, box shows interquartile range and whiskers show range. P value was calculated using two-sided Wilcoxon rank-sum test with cluster bootstrapping to correct inter-eye correlation. d, Correlation between the predictors, baseline SE (upper panel) and PSB at the age of 2 weeks (lower panel), and the outcome (SE at the ages of 2–8 weeks). Scatterplots show 42 individual eyes from 21 animals, regression (lines) and 95% confidence intervals (shaded areas). r values were calculated using Pearson correlation. P values were calculated using F-test against a constant model. Inter-eye correlation was addressed by cluster bootstrapping. e, Predictions of myopia outcomes at the ages of 4 weeks (upper panel) and 8 weeks (lower panel) from the data measured at the age of 2 weeks, using PSB (orange line) and baseline SE (blue line) as predictors. PSB: AUC, 0.89; 95% CI (0.70, 1); SE: AUC, 0.74, 95% CI (0.48, 0.94); Week 8, PSB: AUC, 0.85, 95% CI (0.61, 1); Baseline SE: AUC, 0.73, 95% CI (0.46, 0.95).