Extended Data Fig. 8: Point-of-care design of the FEMMAN assay, and stability of the reagents. | Nature Biomedical Engineering

Extended Data Fig. 8: Point-of-care design of the FEMMAN assay, and stability of the reagents.

From: Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device

Extended Data Fig. 8: Point-of-care design of the FEMMAN assay, and stability of the reagents.

a, Schematic of point-of-care FEMMAN DNA-chip design with Cy5 fluorophore. b, Alpha variant identification in G142-Y145 site by FEMMAN from 10,000 copies/rxn to 1 copy/rxn using Cy5 labelled primer. Stability of Asy-RT-RPA reagents and DNA chip used in FEMMAN for c, IRDye800-SA staining and d, Cy5 labelled primer. The reagents and DNA chip were stored in room temperature for 0 day, 7 days, 14 days, 21 days, and 30 days (from left to right in each column). n = 3 technical replicates, bars represent mean ± s.d.

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