Extended Data Fig. 3: Multiplexed DNA sensing on the FEMMAN chip.
a, Schematic of SARS-CoV-2 viral RNA detection using FEMMAN assay. b, DNA probe layout on FEMMAN chip (sequences listed in Supplementary Table 2). c, IRDye800 fluorescence images for multiplexed DNA microarray when different combination of DNA targets (Target A, B, C & D in Supplementary Table 4) at 200 fM/20 fM were probed. d, Fluorescence image and e, signal quantification of DNA hybridization selectivity test for single base pair (Probe C-1 mismatch) and double base pair mismatch (Probe C-2 mismatch). f, Sequences of the DNA probes and primers for N501 target site. The target site is highlighted in green. g, IRDye800 fluorescence images of a typical titration curve for SARS-CoV-2 wild type viral RNA detection by FEMMAN from 5,000 copies/rxn to 5 copies/rxn on pGOLD. h, Background subtracted fluorescence quantification of viral RNA titration on pGOLD for N501N, N501Y and NTC probes. n = 3 technical replicates, bars represent mean ± s.d.
