Extended Data Fig. 1: Effect of folate-diketone concentration on cell lysis and cytokine release of acute monocytic leukemia and T cell leukemia by the CovCAR T cells in vitro. | Nature Biomedical Engineering

Extended Data Fig. 1: Effect of folate-diketone concentration on cell lysis and cytokine release of acute monocytic leukemia and T cell leukemia by the CovCAR T cells in vitro.

From: Control of the antitumour activity and specificity of CAR T cells via organic adapters covalently tethering the CAR to tumour cells

Extended Data Fig. 1: Effect of folate-diketone concentration on cell lysis and cytokine release of acute monocytic leukemia and T cell leukemia by the CovCAR T cells in vitro.

a, Comparison of FOLR1 and FOLR2 expression by THP-1, THP-1 FOLR1, and THP-1 FOLR2 cells. Open histogram: unstained cells; gray histogram: isotype control; red: folate receptor. b, THP-1 and THP-1 FOLR2 cells were incubated with conventional FOLR2-specific CAR-T cells (m909h-CAR) or in the presence of CovCAR T cells plus different concentrations of folate-diketone prior to analysis of tumor cell lysis (effector:tumour cell ratio 2:1). c, THP-1, THP-1 FOLR1 and THP-1 FOLR2 cells were incubated in the presence of CovCAR T cells plus different concentrations of folate-diketone prior to analysis of tumor cell lysis (effector:tumour cell ratio 5:1). d, CovCAR T cells release IL-2 cytokine in the presence of acute monocytic leukemia cells expressing the folate receptor and folate-diketone in a dose-dependent manner (effector:tumour cell ratio 5:1). Data represent mean ± s.d., n = 4.

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