Extended Data Fig. 10: The rheological properties, in vitro and in vivo degradability, and vascularizing function of degradable zwitterionic gel after SC injection in healthy C57BL/6 mice. | Nature Biomedical Engineering

Extended Data Fig. 10: The rheological properties, in vitro and in vivo degradability, and vascularizing function of degradable zwitterionic gel after SC injection in healthy C57BL/6 mice.

From: An injectable and biodegradable zwitterionic gel for extending the longevity and performance of insulin infusion catheters

Extended Data Fig. 10

a, Photograph of the prepared degradable zwitterionic gel (100 uL). b, Representative micrograph of degradable zwitterionic gel after dispersed in DI water. c, d, Frequency-dependent (c, under 1% strain, 25 °C) and strain-dependent (d, 10 rad/s frequency, 25 °C) oscillatory sweeps of degradable zwitterionic gel. e, In vitro degradation of degradable zwitterionic gel in PBS containing 20 µM GSH (n = 3 independent samples, mean ± s.d.) f, The degradable zwitterionic gel (100 uL) was SC implanted into the mice (n = 5 for each group at each time point). g, h, Representative Masson trichrome staining images (g) and blood vessel staining (brown) using MECA-32 antibody (h) on tissues surrounding SC injected degradable zwitterionic gel at day 30, 60, and 90. Scale bar, 1000 μm in g and 200 μm in h. i, Blood vessel density at different time points post-injection (n = 5 biologically independent samples at each time point, mean ± s.d.). A one-way ANOVA with Tukey multi-comparison was used for statistical analysis.

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