Extended Data Fig. 4: Differentiation of Klg-1 FS mESCs into distinct lineages and cell types. | Nature Biomedical Engineering

Extended Data Fig. 4: Differentiation of Klg-1 FS mESCs into distinct lineages and cell types.

From: Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts

Extended Data Fig. 4: Differentiation of Klg-1 FS mESCs into distinct lineages and cell types.The alternative text for this image may have been generated using AI.

a. IHC showing presence of cells expressing ectoderm (Neurofilament, NF) and mesoderm (Smooth Muscle Actin, SMA), early embryonic lineage markers, in Klg-1 embryoid bodies. b. CD31+ endothelial-like cells with vascular tube-like formations (top) and FOXA2 + , SOX17+ definitive endoderm derived from Klg-1 FS mESCs using directed protocols. c. Troponin+ cells after cardiomyocyte differentiation of Klg-1mESCs, and d. cyclic contraction (top graph) measured as a function of normalized cell displacement and calcium flux (bottom graph) after loading with Fluo-4 AM 15 days after cardiomyocyte differentiation. e. Adipocyte differentiation showing phase contrast, Oil Red O staining, and eGFP fluorescence. The eGFP reporter is expressed by the Pdl1 transgene in Klg-1 FS mESCs shown in Extended Data Fig. 1b).

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