Extended Data Fig. 7: Knockout of FCHO2 and AP2M1 inhibits Omicron Spike-induced syncytium formation. | Nature Biomedical Engineering

Extended Data Fig. 7: Knockout of FCHO2 and AP2M1 inhibits Omicron Spike-induced syncytium formation.

From: High-throughput screening of genetic and cellular drivers of syncytium formation induced by the spike protein of SARS-CoV-2

Extended Data Fig. 7: Knockout of FCHO2 and AP2M1 inhibits Omicron Spike-induced syncytium formation.The alternative text for this image may have been generated using AI.

a-b) Validation of screen hits using sgRNA-directed gene knockouts in A549-ACE2-Cas9 and Vero E6-TMPRSS2-Cas9 receiver cells. The sender cells used to express either WT D614G Spike or Omicron Spike. Data shown are mean ± SD (n = 6 for (a) and n = 3 for (b)). P-values indicated were compared with safe harbor-targeting (a) or non-targeting (b) sgRNA control. Statistical significance was determined using one-way ANOVA. n indicates the number of biological replicates. ****P < 0.0001.

Back to article page