Extended Data Fig. 5: Phenotypic analysis of B7-H3-CAR T cells following stimulation with A549 WT.

B7-H3-CAR+, CAR+Zip2R+, or CAR+Zip7R+ T cells were stimulated twice with A549 WT. CAR positive or CAR and ZipR positive T cells were quantified by flow cytometry pre and post 2nd stimulation (stim) and CD4 and CD8 positive T cells post 2nd stim (N = 2 donors). a, Percent CAR+ or CAR+ZipR+ cells pre and post 2nd stim (***p = 0.0005, two-tailed t-test). b, Percent CD4+ (left panel) or CD8+ (right panel) cells post 2nd stimulation (**p = 0.001, ***p = 0.0003, two-tailed t-test).