Extended Data Fig. 5: Somatic hypermutation in B cells engineered with native-loci and intron-targeting approaches.
From: In vivo affinity maturation of mouse B cells reprogrammed to express human antibodies
a, Figure similar to that in Fig. 3c except that results from the indicated antibodies are presented separately. Mean ± s.d, n = 3 (Native) n = 2 (Intron) mice. **p = 0.0030; *p = 0.0387; *p = 0.0106; *p = 0.0466; Two-way ANOVA with Šídák’s multiple comparisons. b, Figure similar to that in Fig. 3d except that results from the indicated antibodies are presented separately. Horizontal lines indicate mean values. Significance was determined by two-way ANOVA with Šídák’s multiple comparisons and indicated from left to right: ***p = 0.0008; nsp = 0.5330; nsp = 0.5116; nsp = 0.9922. c, The percent of amino-acid mutations in each heavy-chain and light-chain CDR and framework (FR) region from native loci- and intron-edited B cells is indicated for each mouse. Horizontal line indicates the average number of mutations found in each region. **p = 0.0056; *p = 0.0232; **p = 0.0018; Two-way ANOVA with Šídák’s multiple comparisons.
