Extended Data Fig. 10: Characterization of SARS-CoV-2 antibody variants.
From: In vivo affinity maturation of mouse B cells reprogrammed to express human antibodies
a, Figure shows the frequency of heavy-chain and light-chain amino-acid substitutions at each ZCB11 and S309 residue, observed in cells isolated from three native-loci-edited mice. Grey indicates heavy-chain and light-chain CDRs. SHM levels in electroporated cells cultured ex vivo without in vivo evolution are indicated with dotted lines. b, Sequences of wild-type ZCB11 heavy and light chains and their variants (top) and S309 chains and their variants (bottom), characterized in Fig. 7d, are provided. Changes from wild type are indicated in red. The affinity of these variants for the BA.5 S protein (ZCB11 variants) and XBB.1.5 RBD (S309 variants), are shown at the right of each sequence. c, Representative curves comparing S309 (grey) and S309-v13 (green) neutralization against SARS-CoV-1 and the indicated SARS-CoV-2 variants. Mean ± s.e.m, n = 3 technical replicates. A summary of IC50 values against a broader panel of variants is provided in Fig. 7g.
