Extended Data Fig. 3: Treatment with CAMP-carrying neutrophils elicits a systemic immune response in the B16F10 model.

a, Experimental scheme for immunophenotyping. B16F10-tumour-bearing mice were treated with two doses of Saline, Neutrophil only (NE) or neutrophil-CAMPs (NE + CAMP). Blood, tumours, tumour-draining lymph nodes and spleens were harvested 48 hours after the second dose and analysed using flow cytometry. b, Neutrophil (%Ly6G⁺ Ly6C⁺ of CD45⁺) accumulation in the spleen was higher in the NE + CAMP group. c, Neutrophil accumulation in the tumour-draining lymph nodes was higher in the NE + CAMP group. d, CD8 + T cells in the spleen displayed higher expression of PD-1 in NE + CAMP group. e-f, NK cells (e) and CD8 + T cells (f) in the spleen displayed higher expression of LAMP-1 in the NE + CAMP group. g, The proportion of CD8+ effector memory T cells in the spleen was reduced in the NE + CAMP group. h, The proportion of CD8+ effector memory T cells in the tumour was marginally increased in the NE + CAMP group. i, CD8 + T cells in the blood in the NE + CAMP groups displayed activated phenotype as seen from the higher expression of PD-1. j-k, CD8 + T cells (j) and NK cells (k) in the tumours in the NE + CAMP group displayed modest activation with a marginally higher expression of CD69. l, Marginally higher accumulation of neutrophils in the tumours was observed in NE + CAMP group. n = 6 biological replicates for saline, n = 8 biological replicates for NE, n = 8 biological replicates for NE + CAMP. All data are represented as mean ± SEM. Sentences at the top of each panel represent the key observation that pertains to the NE + CAMP group. Data analysed using Ordinary one-way ANOVA with Tukey’s multiple comparisons test.