Extended Data Fig. 10: Extended histology data from BLUsH and control experiments.

(a) Data corresponding to Fig. 4d, showing BLUsH MRI maps (left), GLucM23 staining (blue), bPAC staining (red), and merged images for three individual rats examined by postmortem histology. (b) Analogous MRI and histology data from three animals that received control viruses in place of BLUsH components. The control treatment results in vascular tdTomato expression (red) in place of bPAC. AAV-directed GLucM23 delivery is identical between BLUsH and control animals and results in robust expression (blue) without corresponding MRI signal changes (left).