Extended Data Fig. 4: T-cell differentiation and exhaustion phenotypes from the microbioreactor and the gas-permeable well plate. | Nature Biomedical Engineering

Extended Data Fig. 4: T-cell differentiation and exhaustion phenotypes from the microbioreactor and the gas-permeable well plate.

From: A high-density microfluidic bioreactor for the automated manufacturing of CAR T cells

Extended Data Fig. 4: T-cell differentiation and exhaustion phenotypes from the microbioreactor and the gas-permeable well plate.The alternative text for this image may have been generated using AI.

a–e, Cells were sampled on Days 6 and 12, and the percentage of CCR7+ CD45RA+ (naïve and stem cell memory, Tn/Tscm), CCR7+ CD45RA– (central memory, Tcm), CCR7– CD45RA– (effector memory, Tem), and CCR7– CD45RA+ (terminally differentiated effector, Temra) cells gated on CAR+ live cells (a), on CD4+ cells (b), on CAR+ CD4+ cells (c), on CD8+ cells (d), and on CAR+ CD8+ cells (e) were quantified by flow cytometry. Data shown are mean ± SD of n = 3 technical replicate wells/cassettes per donor. f–g, Cells were sampled on Days 6 and 12, and the percentage of total live cells expressing CD127 (f) and CD57 (g) were quantified by flow cytometry. Data shown are individual values and mean ± SD of n = 3 technical replicate wells/cassettes per donor. P values were determined using two-tailed unpaired t-tests (df=4).

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