Extended Data Fig. 1: Protegin-1 dmSLAY library diversity and sequencing analysis. | Nature Biomedical Engineering

Extended Data Fig. 1: Protegin-1 dmSLAY library diversity and sequencing analysis.

From: Deep mutational scanning and machine learning for the analysis of antimicrobial-peptide features driving membrane selectivity

Extended Data Fig. 1

a, an alanine scan performed on the native Protegrin-1 (PG-1.0) amino acid sequence showing antibacterial activity (MIC) in µg/ml. Reported MIC is the median of triplicate reactions. b, Chart of the sequence variance found within the Protegrin-1 dmSLAY library. The native Protegrin-1 sequence is shown at the top with mutations observed at each location within the library below. Amino acids are color coded by side chain similarity. Brackets represent where disulfide bonds are present. c, Principal Component Analysis of the overall induced and uninduced triplicate sample variance. d, ROC curve with different MIC cut offs for active and inactivity for log2-fold change cut off across a range of log2-fold change scores (L2FC).

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